Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis
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چکیده
منابع مشابه
Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari: Ixodidae).
The success of immunological method for the control of ticks depend on the use of potential key antigens as tick vaccine candidates. Chitinase is induced by ecdysteroids to degrade the older chitin at the time of molting. Previously, we cloned a gene encoding 113 kDa protein (CHT1) of Haemaphysalis longicornis, and identified the CHT1 as a protein of chitinase (You et al. 2003). In this study, ...
متن کاملIdentification and characterization of the plasma kallikrein-kinin system inhibitor, haemaphysalin, from hard tick, Haemaphysalis longicornis.
The plasma kallikrein-kinin system inhibitor, haemaphysalin, from the hard tick, Haemaphysalis longicornis, was identified. It was found that haemaphysalin inhibited activation of the plasma kallikrein-kinin system by interfering with reciprocal activation between factor XII and prekallikrein. It did not, however, inhibit amidolytic activities of factor XIIa and kallikrein. Direct binding assay...
متن کاملProteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae)
Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed...
متن کاملEpidemiology of toxoplasmosis: role of the tick Haemaphysalis longicornis
BACKGROUND Toxoplasma gondii infection is mainly caused by ingestion of water or food that is contaminated with oocysts excreted by cats, or by eating raw meat containing T. gondii tissue cysts. However, oral transmission does not explain the common occurrence of toxoplasmosis in a variety of hosts, such as herbivorous animals, birds, and wild rodents. Little information exists on the maintenan...
متن کاملIdentification of genes encoding cement-like antigens expressed in the salivary glands of Haemaphysalis longicornis.
A cDNA expression library from the salivary glands of hard tick, Haemaphysalis longicornis, was constructed. Immunoscreening was performed using sera of the rabbit repeatedly infested with ticks and seventeen positive clones were obtained. A BLASTP search suggested that 8 sequences matched with that of hypothetical H. longicornis sequence and one clone encoded HL35 antigen U from the same tick ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2003
ISSN: 0014-2956,1432-1033
DOI: 10.1046/j.1432-1033.2003.03560.x